Enhancement of Sensitivity of Immunofixation Protein Electrophoresis Methods

Wednesday, July 31
6:00 p.m. - 7:30 p.m.
Location: Hyatt Regency McCormick Place
Room: Hyde Park A
Sponsored by Helena Labratories


Techniques to enhance the sensitivity of immunofixation electrophoresis for serum and urine will be described along with the evidence for improvement in detecting monoclonal immunoglobulins in serum and urine. Emphasis will be placed on using undiluted serum, concentrated urine, use of antisera to free immunoglobulin light chains, and immune subtraction. Modifications to the procedure will be covered in detail so that any laboratory performing protein electrophoresis could execute the procedures without the need to acquire any additional equipment or the need for additional personnel or expertise. The modified methods compare favorably with the sensitivity of mass spectroscopy.


Robert S. Galen, MD MPH
Professor Emeritus Epidemiology and Biostatistics, University of Georgia College of Public Health


Gurmukh Singh, MD, PhD
Shepeard Chair in Clinical Pathology, Augusta University